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Grenoble INP - UGA
Physico chemistry of solids, thin films, biotechnologies
Applications for micro & nano- technologies, energy, health ...

> Research > IMBM

Bone tissue engineering

OBJECTIVES

Our initial studies showed that bone morphogenetic proteins (BMPs) can be presented to cells in a matrix-bound mannerr via a biomaterial surface (Crouzier et al, Small 2009) . This biomaterial is a biomimetic film, made by self-assembly of polysaccharides and polypeptides, which can trap the BMPs.
We then proved that 1) BMP-coated ceramic granules are osteoinductive in an ectopic site when implanted in rat muscle  (Guillot et al, Biomaterials 2011) 2) BMP-2-loaded film-coated titanium implants can be dried and sterlized while remaining osteoinductive (Guillot et al, Biomaterials 2013) . In 2016, we showed that a critical size femoral bone defect in rats can be regenerated by combining an initially empty 3D polymeric scaffold made of to the osteoinductive film coating. The newly formed bone contained cortical and trabecular bones and it was vascularized (Bouyer et al, Biomaterials 2016).
In collaboration with Prof. Georges Bettega (CHR Annecy and IAB Grenoble), we are continuing our pre-clinical developments in large animals in the frame of the ERC POC REGENERBONE (PI CPicart) , Fondation Gueules Cassées and ANR OBOE 2019-2022 (PI CPicart).
 

Researchers & Technical staff

   Catherine Picart (CEA Research Director)
   Paul Machillot (CNRS Assistant Engineer)





 

Students

   Charlotte Garot (PhD student)
   Michael Bouyer (PhD student)
   Sanela MORAND (M2 clinician)

Selected Publications

Bone regeneration strategies: Engineered scaffolds, bioactive molecules, and stem cells: current stage and future perspectives. 

Ho-Shui-Ling, A., Bolander, J., Rustom, LE., Johnsson AW., Luyten, FP., and C. Picart.  Biomaterials 2018.

Solvent-free preparation of porous poly(L-lactide) microcarriers for cell culture

Kuterbekov, M. Machillot, P., Lhuissier, P., Picart, C., Jonas, AM., Glinel, K. Acta Biomaterialia 2018.


 
   

Surface delivery of tunable doses of BMP-2 from a polymeric scaffold induces volumetric bone regeneration

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Bouyer M, Guillot R, Lavaud J, Plettinx C, Olivier C, Curry V, Boutonnat J, Coll JL, Peyrin F, Josserand V, Bettega G, Picart C. Surface delivery of tunable doses of BMP-2 from an adaptable polymeric scaffold induces volumetric bone regeneration. Biomaterials. 104:168-81, 2016. 

   

Assessment of a polyelectrolyte multilayer film coating loaded with BMP-2 on titanium and PEEK implants in the rabbit femoral condyle

 Guillot R, Pignot-Paintrand I, Lavaud J, Decambron A, Bourgeois E, Josserand V, Logeart-Avramoglou D, Viguier E, Picart C.  Acta Biomaterialia 2016.

 
Spatio-temporal control of LbL films for biomedical applications: from 2D to 3D.
Monge C, Almodóvar J, Boudou T, Picart C.
Adv. Healthc. Mater. 2015.
Spatial patterning of BMP-2 and BMP-7 on biopolymer films and the guidance of muscle cell fate. 
Almodovar J, Guillot R, Monge C, Vollaire J, Seilimovic S, Coll JL,
Khademhosseini A, Picart C.
Biomaterials. 2014.
The stability of BMP loaded polyelectrolyte multilayer coatings on titanium.
Guillot R, Gilde F, Becquart P, Sailhan F, Lapeyrere A, Logeart-Avramoglou D, 
Picart C.
Biomaterials. 2013.
Secondary structure of rhBMP-2 in a protective biopolymeric carrier material.
Gilde F, Maniti O, Guillot R, Mano JF, Logeart-Avramoglou D, Sailhan F, Picart C.
Biomacromolecules. 2012.
The performance of BMP-2 loaded TCP/HAP porous ceramics with a polyelectrolyte multilayer film coating.
Crouzier T, Sailhan F, Becquart P, Guillot R, Logeart-Avramoglou D, Picart C.
Biomaterials. 2011.
Presentation of BMP-2 from a soft biopolymeric film unveils its activity on cell adhesion and migration.
Crouzier T, Fourel L, Boudou T, Albiges-Rizo C, Picart C. 
Adv. Mat. 2011.
Layer-by-layer films as a biomimetic reservoir for rhBMP2 delivery : controlled differentiation of myoblasts to osteoblasts.
Crouzier T, Ren K, Nicolas C, Roy C, Picart C.
Small. 2009.

Date of update November 8, 2019

Université Grenoble Alpes