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Thesis defence by Monica VALLEJO PEREZ

Published on June 23, 2021
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PhD Defense July 12, 2021
14 p.m. - Amphi M 001, ground floor, building M
Phelma Minatec
Grenoble INP - Phelma
3 parvis Louis Néel - 38000 Grenoble
Accès : TRAM B arrêt Cité internationale
Free entrance - No registration

“Development of biosensors based on randomly oriented silicon nanowire networks for electrochemical or field-effect detection”

Monica VALLEJO PEREZ

Monica VALLEJO PEREZ


Keywords:
Silicon nanonet, electrical detection, electrochemical detection, biofunctionalization, DNA hybridization, thrombin



cliquer pour voir la liste des membres du jury/clic here for the jury members

 

Abstract


Randomly oriented nanowire networks (nanonets) exhibit interesting properties, arising from the intrinsic properties of the individual nanostructures, but also offer remarkable new properties related to the nanonet structure, namely a better reproducibility, and high fault tolerance and flexibility. In this context, this project investigated the possibility of using a 2D assembly of randomly oriented Si nanowires (Si nanonet), as building block for the fabrication and development of two different types of biosensors: Field-effect transistor (FET) biosensors for the electrical detection of DNA hybridization, and electrochemical biosensors for thrombin detection.

For this purpose, the development of each type of sensor was divided into three parts: (i) fabrication of the Si nanonet-based device, (ii) biofunctionalization of the device, allowing the grafting of the bioreceptor (TBA-15 aptamer), and (iii) detection of the target molecule.

Regarding the Si nanonet-based FET biosensors, the work focused on the implementation and the optimization of a biofunctionalization protocol, enabling the proper immobilization of the bioreceptor on the surface of the Si nanonet-based FETs. Then, the ability to electrically detect the DNA hybridization in dry environment was studied. Concerning the electrochemical biosensors, it was developed for the first time a process allowing the fabrication and biofunctionalization of a 2D Si nanonet-based biosensor, enabling thrombin detection in nM concentrations, which indicate either the risk or the presence of thrombosis. These promising results open the door to future projects implying the use of 2D Si nanonet-based devices in the field of biodetection.

Membres du jury/ Jury members :
 

Dr.

Hafsa Korri-Youssoufi

Institut de Chimie Moléculaire et des Matériaux d’Orsay, CNRS, Orsay (France)

Rapporteuse

Prof.

Benoît Piro

Université Paris-Diderot, Paris (France)

Rapporteur

Dr.

Michael Holzinger

Département de Chimie Moléculaire, CNRS, Grenoble (France)

Examinateur

Prof.

Thierry Noguer

Université de Perpignan, Perpignan (France)

Examinateur

Dr.

Valérie Stambouli

Laboratoire des Matériaux et du Génie Physique, CNRS, Grenoble (France)

Directrice de thèse

Dr.

Nicolas Spinelli

Département de Chimie Moléculaire, CNRS, Grenoble (France)

Co-encadrant de thèse, invité

Dr.

Céline Ternon

Laboratoire des Matériaux et du Génie Physique, CNRS, Grenoble (France)

Co-encadrante de thèse, invitée

Dr.

Chantal Gondran

Département de Chimie Moléculaire, CNRS, Grenoble (France)

Invitée


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Date of update June 30, 2021

Université Grenoble Alpes