Interactions between biomolecules (therapeutic proteins, nucleic acids) and the materials of the containers in which they are produced, stored and used raise issues concerning the stability of therapeutic formulations and the performance of medical devices. We study the structural conformation and aggregation of therapeutic proteins at interfaces. We analyze the phenomena of adsorption/desorption and molecular competition (proteins, surfactants, etc.) on material surfaces. We exploit the interaction of biomolecules with materials in order to optimize medical diagnosis and therapy strategies. To measure these phenomena at interfaces, we use interface-sensitive methods (SPRi, QCMd, IR) and develop new methods for in situ quantification of protein adsorption on medical and other materials.
Permanent Staff
Non permanent staff
10 selected publications
[1] Frachon T, Bruckert F, Le Masne Q, Monnin E and Weidenhaupt M (2016) Insulin aggregation at a dynamic solid-liquid-air interface. Langmuir 32(49), 13009-13019 https://doi.org/10.1021/acs.langmuir.6b03314
[2] Lefebvre G, Maze A, Alvarez-Palencia Jimenez R, Bruckert F, Filipe V, Huille S and Weidenhaupt M (2021) Surfactant Protection Efficacy at Surfaces Varies with the Nature of Hydrophobic Materials. Pharm Res 38, 2157-66
DOI:10.1007/s11095-021-03133-6
[3] Alvarez-Palencia Jimenez R, Maze A, Vian G, Bruckert F, Bensaid F, El-Kechai N, Weidenhaupt M (2024) Development of an ELISA-based device to quantify antibody adsorption directly on medical plastic surfaces. EJPBP, 203, 114425,
https://doi.org/10.1016/j.ejpb.2024.114425
[4] Alvarez-Palencia Jimenez R, Maze A, Bruckert F, Bensaid F, El-Kechai N, Weidenhaupt M (2024) Evaluating surfactant effectiveness in preventing antibody adsorption directly on medical surfaces using a novel device, EJPBP, 205, 114539
https://doi.org/10.1016/j.ejpb.2024.114539
[5] Orsini E, Bruckert F, Weidenhaupt M, Cugat O, Kauffmann P, Delshadi S (2025) Magnetically localized detection of amplified DNA using biotinylated and fluorescent primers and magnetic nanoparticles, Biosensors, 15, 195
https://doi.org/10.3390/bios15030195
Projects
MagMol, NabioS- MagIA Diagnostics, Pack Ambition recherche Région AURA, 2022-2026
MagPhor, NaBioS- G2Elab, FITInnovE Région AURA, 2025-2028
AMI-IBES, Innovative Biohealth Education School, ANR-23-CMAS-0009, 2023-2028
National and international Collaborations
- G2Elab
- Becton Dickinson,
- MagIA Diagnositcs,
- Departement Pharmacie UGA