Séminaire Interne au LMGP - 27/09/2016 - Benoît Kalman

Benoît KALMAN - Phd Student - IMBM  

Abstract

Actual in vitro models for skeletal muscle tissue engineering present many limitations for high-content screening analysis. Two-dimensional (2D) models do not mimic the native cellular microenvironment (Monge et al. 2012), whereas 3D models require large amounts of cells, thus limiting the reproducibilityof experiments (Chiron et al. 2012; Madden et al. 2015).

In our lab we developed two innovative technics based on microtechnologies to study skeletal muscle tissue. The first one consist in a PDMS platform structured by microgrooves that allows to align tridimensional myotubes and study myogenesis. The second one is a microfabricated platform that enables the generation of arrays of 3D microtissues and give direct access to skeletal muscle contractility.

Here we will introduce those tools and show how we succeeded in generating and studying 3D aligned human myotubes and 3D microtissues from human myoblast.