Protein interactions with biomimetic lipid membranes
The aim of this project is to understand at the molecular level the link between membrane structures and the actin cytoskeleton. Ezrin and Moesin, members of the ezrin/radixin/moesin (ERM) family, are membrane/cytoskeleton linkers involved in cell morphogenesis and motility. Our goal is to study ERM proteins/lipid membrane/cytoskeleton interactions in vitro in biomimetic systems in order to better understand the underlying molecular processes. To this end, we produce recombinant ERM proteins and mutants and engineer biomimetic lipid membranes, especially LUVs (large unilamellar vesicles) and supported lipid bilayers (SLBs). Molecular interactions are studied by a large set of biophysical techniques (QCM-D, AFM, FTIR spectroscopy, fluorescence spectroscopy, and TIRF).